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KMID : 0360919630060070737
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Journal of the Korean Medical Association
1963 Volume.6 No. 7 p.737 ~ p.744
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Total Protein, Total Cholestrol, Phospholipids Level and Transaminase Activities of Various Organ Homegenates in Normal and Liver-dameged Rabbits in Relation to the Serum Levels
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金丁龍/Kim, Chung Yong
李世郁/姜文聖/崔圭完/李元魯/Lee, Seh Ook/Kang, Moon Sung/Choi, Kyoo Wan/Lee, Won Ro
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Abstract
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Much investigations concerning the relationship between cholesterol amounts in the arteries and pypercholesterolemia were performed. There might be big deposits in the arteries and possibly in the tendons, but this does not necessarily mean that concentrations in other tissues are high.
Few reports could be seen on the changes of the contents such as protein, lipids and enzymes of the various organs in the cases of damaged liver. The authors, then, determined the concentrations of total protein, total cholesterol, phospholipid levels and transtminase activities in serum and various tissue homogenates to investigate the normal concentrations and changes of those contents in livel-damged states relation to its serunl levels.
In this experiment, 18 normal rabbits, 9 partial1y hepatectormized rabbits that lived for 1 week after resected two lobes of the liver and 18 rabbits with the damaged ]ive induced by long term (12 mooths)administration of large doses (10 gm. daily of red pepper were used. The tissue materials were treated as follows: for the wet tissues analysis, 0.5 mg. of the liver, train gray and white matter kidney cortex, adrenal gland, heart muscle, lung, skeletal muscle and spleen has been weighed in tile still wet state. These were then homogenized in 10 cc. of distilled water in a Thomas´No. 8430 homogerlizator with 1,000 r. p. m. for 20 minutes until no big particles could be seen microscopically (microscopic survey showed only isolated intact cells). The homoghnizates were quantitatively transfered for the detrmlinations of the contenis of total potein total choleselol phospholipid levels and transanlinase activities in various tissues noticed above.
The results of experiment are summarized as follows:
1) There were no big differences in the content of total proteinl among various organs and tissues. The concentration of total cholesterol was significantly high in the brain white matter and adrenal gland, low in the skeletal muscle. And the concentration of phospholipid were the highest in the brain white matter and the lowest in the skeletal muscle and adrenal gland.
The highest concentration of GO·-Transaminase in the liver and cardiac muscle, and the GP-tansalninase in the liver were observed. In the spleen and lung the contents of transaminase were the lowest The comparison of contents in various organs and
tissues were illustrated in figure 1 and 2.
2) No significant changes of the concentration of total protein in various organs and tissues were observed among partially hepatectmoized and liver damaged rabbits as in the serum total protein. The concentration of total cholestrol of various organs and tissues was a little increased in the in the serum total cholesterol was highly increased in the partially hepatectomized and livel-damaged rabbit. The concentration of phospholpld was much decreased in the liver but increased in serum in the liver-damaged rabbits, and in the other organs and tissues the decrease was not so great. GO-tansaminase was increased in the serum of the liver-damaged rabbits, on the other hand decreased significantly in the liver and cardiac muscle. GP-transaminase was also increased in the serum of the liver-damaged rabbits slightly. but significant decrease in the liver and not so great change in the other organs and tissues were observed.
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KEYWORD
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